Queen's Laboratory for Molecular Pathology (QLMP)


Queen's Laboratory for Molecular Pathology

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Immunohistochemistry (IHC) and Immunofluorescence (IF) staining:

  • CD20 Immunohistochemistry stain

    CD20 immunohistochemistry stain of tonsil tissue

  • BCL2 Immunofluorescence

    Immunofluorescence staining of Bcl2 proteins in HeLa cells.  Counterstained with DAPI.

  • CD31 immunohistochemistry

    CD31 immunohistochemistry staining of mouse lung tissue

  • CD68 Immunofluorescence

    CD68 Immunofluorescence staining in HeLa cells.  Counterstained with DAPI.

  • K1-67 immunohistochemistry

    K1-67 immunohistochemistry of human breast carcinoma

We can provide customized immunohistochemistry and immunofluorescence services:

  • Consultation to assess staining strategies.
  • Chromogenic or fluorescent detection methods offered
  • Multiplex staining available

                            Detailed flow chart of the immunohistochemistry and immunfluorescence process from initiation > collection of materials > trial run > experiment

Thinking of trying a custom antibody?  Why not take advantage of QLMP Immunostaining Facility and let us do the troubleshooting!  We offer free initial consultation and set-up.  In all cases the investigator must supply the antibodies and robust positive and negative controls, as immunostaining may generate false negative and false positive results; unless well-validated antibodies are available for the target of interest.  Our IHC/IF specialist Lee Boudreau has prepared a helpful document to explain the basic principles, it is found below.  Also included is a pamphlet about the Ventana Discovery XT

Principles of IHC from QLMP (PDF, 10MB)

Ventana User Guide (PDF, 608KB)